模型信息
中文名称:SARS-CoV 滴鼻感染BALB/c小鼠模型
英文名称:SARS-CoV intranasally infection of BALB/c mouse model
类型:SARS-CoV感染动物模型
分级:NA
用途:用于SARS-CoV感染研究。
研制单位:日本国立传染病研究所@@https://www.niid.go.jp/
保存单位:日本国立传染病研究所@@https://www.niid.go.jp/
研究背景
暂无介绍
制备方法
BALB/c mice (female, 6-week-old (Japan SLC, Shizuoka, Japan); n = 6–7; total, 25) were immunized subcutaneously twice at 2 week intervals with 1 µg, 0.5 µg, 0.1 µg, or 0.05 µg doses of the protein in 100 µL PBS.Approximately 3 weeks after the second immunization, mice were anesthetized via intraperitoneal injection of a mixture of 1.0 mg ketamine (Daiichi Sankyo Company, Tokyo, Japan) and 0.02 mg xylazine (Byer Japan, Osaka, Japan) (0.08 ml/10 g of body weight). These mice were then inoculated intranasally with SARS-CoV (106 TCID50 in 30 µl of 2% FBS-MEM).
评价验证
暂无介绍
生物安全性
暂无介绍
讨论与结论
血管周围水肿,炎症细胞浸润
了解中析
实验室仪器
合作客户
